Binding affinity analysis of the interaction between Homer EVH domain and ryanodine receptor with biosensors based on imaging ellipsometry
The interaction between the Homer EVH domain and RyR plays an important role in calcium signaling channels in the heart and the skeletal muscles. A biosensor based on imaging ellipsometry was used to analyze the binding affinity between the Homer EVH domain and RyR. The results confirm that the Homer EVH domain can recognize RyR specifically with high binding affinity of approximately 10(-8) M. This information will contribute to understanding of the role of the interaction between the Homer EVH domain and RyR in intracellular calcium channels.
The authors acknowledge the financial support of the International Science & Technology Cooperation Program of China (2015DFG32390), the 7th Sino-Portugal Scientific and Technological Cooperation of 2013-2015, the National Basic Research Program of China (2015CB352100), and the National Natural Science Foundation of China (21305147) and (81472941).